Advances in Tracer Methodology: Volume 2 A collection of - download pdf or read online
By Martin D. Kamen (auth.), Seymour Rothchild (eds.)
Advances in Tracer technique, quantity 2, documents the professional ceedings of the sixth, seventh, and eighth Symposia on Advances in Tracer technique. those Symposia, that are a part of a continuous sequence subsidized by means of the recent England Nuclear Corp. and the Packard device corporation, Inc., are dedicated to the total isotope tracer box: training and research of classified compounds, functions within the chemical, biochemical, and scientific fields, and future health physics concerns linked to tracer paintings. The papers during this quantity mirror convinced developments that are famous within the tracer box: expanding reliance on biochemical equipment for labeling esoteric compounds, becoming understanding of the issues of radiation decomposition, using complicated instrumentation for the research and detection of radioactive substrates, and the speedily growing to be program of tracer compounds to medical analysis. the 3 Symposia at which the papers have been awarded have been hung on the next dates: DATE place CHAIRMAN 11/16/62 big apple urban Daniel Steinberg, leader Laboratory of Ce1lular body structure and Metabolism, nationwide middle Institute, Bethesda, Maryland 3/8/63 la Martin Kamen collage of California San Diego, California 11/8/63 Chicago Joseph L. Rabinowitz Veterans management health facility Philadelphia, Pennsylvania once more, it's the editor's excitement to recognize the aid of his colleagues in making plans and working a few of the Symposia, in addition to the superb cooperation of the chairmen, who acquitted themselves of their appointed initiatives in a most productive demeanour, With humor and realizing. S. R. Boston, Mass.
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Additional info for Advances in Tracer Methodology: Volume 2 A collection of papers presented at the Sixth, Seventh, and Eight Symposia on Tracer Methodology plus other papers selected by the editor
Reflux condenser the outlet of which passed through a U -tube filled with potassium hydroxide pellets. The outlet of the U-tube was connected to two traps in series. These traps each held 25 ml of solvent through which the diazo methane was bubbled. The traps were cooled in a dry ice-acetone mixture. Connections where necessary were made with rubber stoppers so that the diazomethane did not come in contact with ground-glass fittings. M. A. Muhs, E. L. Bastin, and B. E. 28 g (20 mmoles) of N-methyl-C 14 -N-nitroso-p-toluenesulfonamide in 10 ml of anisble.
21. • Luson, N.. , J. BioI. Chern. 159:475 (1945). 22. Arnstein, H. R. V.. and Bentley, R .. Biochem. J. 54:493 (1953). 23. Abraham. S .. and Chaikoff. I. , Arch. Biochem. Biophys. 41:143 (1952). 24. Abraham, S.. Chaikoff. I. , and Hassid, W. , J. BioI. Chern. 195:567 (1952). 25. Ashmore, J .. Hastings, A. • Nesbett, F. B .. and Renold, A. , Proc. Nat. Acad. Sci. S. 40:673 (1954). 26. McIlwain, H .. and Buddie. H. L.. Biochem . T. 53:412 (1953). 27. Krebs, H. , Z. Physiol. Chern. 210:33 (1932), 28.
Three grams of freshly prepared liver slices were added, the plunger of the syringe inserted, and the air gently expressed through the polyethylene tube after removal of the clamp. The KHC 14 0 3 solution was then introduced into the reaction syringe containing the medium and slices, through the polyethylene tubing which attached the two syringes. Forty ml of a mixture of 95% O2 and 5% CO 2 gas was added to the KHC 14 0 3 syringe and this allowed to flow into the reaction syringe which was then "clamped.
Advances in Tracer Methodology: Volume 2 A collection of papers presented at the Sixth, Seventh, and Eight Symposia on Tracer Methodology plus other papers selected by the editor by Martin D. Kamen (auth.), Seymour Rothchild (eds.)